Bioactive constituent of Delphinium denudatum Wall. and their Antioxidant efficacy

Delphinium denudatum known as Jadwar, is critically enlarged Himalayan herbs, which was once commonest species. It is distinguished by its relatively small blue or violet color and it is one of the important drugs used as indigenous medicine in India, especially in Unani medicine system. A number of studies have been done on its phytochemical and pharmacological properties. Bioactive constituent were isolated from the petroleum ether-soluble fraction of root of Delphinium denudatum and their structures were elucidated as β-Sitosterol based on mass and nuclear magnetic resonance (NMR) spectroscopy. Antioxidant activity of β-Sitosterol was evaluated through DPPH radical scavenging method and it revealed that β-Sitosterol was shown to trap free radicals in a concentration dependent manner as high as 65.02% using 160 μg/mL.